Proteins - the natural biopolymers (1985)

Documentary №53741, 3 parts, duration: 0:27:32
Production: Centrnauchfilm (CNF)
Director:Chernyavskij L.
Screenwriters:Trishina B.
Camera operators:Ziljberg L., Popov N., Yakshin M.

Annotation:

The film is recommended by the Ministry of Higher and Secondary Special Education of the USSR for use as a teaching tool. The film analyzes of protein compounds are affected, the problem of protein biosynthesis.

Reel №1

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Schedule XVI Conference of the Federation of European Biochemical Societies (FEBS), past the booth is a man.

A fragment of an interview with A.S.Spirinym.

On the tables of participants water bottles, pamphlets, flags.

Scientists communicate in between meetings.

Scientist at the podium.

People in the exhibition.

Laboratory experimentation.

Photos on the stands (view through the glass).

Drawing.

Flasks and retorts on a background of drawings and photographs.

Portraits of scientists.

Says French scientist Zh.

Etel.

Alternation: scientists at the forum, demonstration experiments.

Institute of Protein Research.

Laboratory experiments were conducted (shooting from the street through a window).

Scientist walks down the hall and invites journalists to the laboratory.

Entry to the laboratory of protein biosynthesis.

The scientist walks into a room, at a table working laboratory.

A man reading documents.

Experiments on extracting protein.

Cutting the biological material.

Milling.

Transfusion protein solution.

Precipitate the proteins in solution using ammonium sulfate.

Dry the precipitate at the bottom of the flask.

Stages isolation of pure protein using biuret reaction.

Method of high speed centrifugation.

Proteins were transferred to a test tube.

The tube was placed in the rotor.

The rotor is inserted into a centrifuge.

The rotor rotates.

The separated proteins in vitro.

Removing proteins.

Scientist among devices.

Demonstration of chromatography methods.

Preparation of protein in the dry state by lyophilization (freeze-drying).

Personnel:

Spirin AS - Scientist, biochemist, doctor of biological sciences, academician, director of the Institute for Protein Research.

Calendar: 06.1984

Locations: Moscow [820] Moscow region [788]

Reel №2

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View of the street from the hall of the institute.

The corridors are people (in the shadow of the doorway lab structural analysis).

The door opens.

The laboratory operates V.V.Filimonov.

The scientist is part of a laboratory study of the chemical structure of the protein.

The team of scientists builds on the circuit board of the primary structure of the protein.

Scientists are discussing the work.

Man breaks hen egg.

Animation: a cracked egg protein together with the flow of formula.

Animation: the formula of amino acids, the formation of peptide bonds.

The scientist is a scheme of the primary structure of the protein.

Experiments on the hydrolysis of proteins.

Determination of the amount of acids in the hydrolyzate using an automatic amino acid analyzer.

Results of analysis on a computer.

Determining the order of the amino acid compounds (preparation of the primary structure of the protein).

Cleavage of amino acids.

Determining the types of amino acids by chromatography.

Printouts from the research results.

Animation: polypeptide chain amino acids, alpha-helix formation of secondary bonds, the formation of the tertiary structure of the protein.

Conference participants discussed the structure of the protein as an example of the layout.

A fragment of an interview with B.K.Vaynshteynom.

X-ray structural studies of protein crystals.

The data on the computer screen.

Animation: the formation of a three-dimensional structure of the protein on the basis of mathematical calculations.

Scientists build molecular layout.

Personnel:

Weinstein BK - Physicist, Corresponding Member of the USSR Academy of Sciences, academician of the USSR Academy of Sciences. Filimonov VV - Candidate of Physical and Mathematical Sciences.

Calendar: 06.1984

Locations: Moscow [820] Moscow region [788]

Reel №3

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A fragment of an interview with A.S.Spirinym.

Animation: the three-dimensional structure of the protein.

Man breaks hen egg.

Denaturation of the protein in a solution of hydrochloric acid (to record images experiences added Dali painting "Geopolitical baby" with the change of the color spectrum).

Animation: the three-dimensional structure of a protein is washed away and melts.

On the grid is broken shells, walks next to the chicken.

Tells V.V.Filimonov.

Microorganisms under microscope.

Animation: protein compounds are painted in different colors.

Ciliate-shoes under the microscope.

Alternation: proteins in different colors of the spectrum (multrabota), hairy caterpillar on a branch, sea turtle, young crocodiles, elephant crosses the glade.

Microorganisms under microscope.

Germinating grass.

It dissolves the foliage.

Chamomile blossoms.

Disclosed buds of poppies.

Animation: the structure of the ribosome.

A fragment of an interview with A.S.Spirinym.

People talking in the hallway.

Scientists working in a laboratory.

Salvador Dali painting "Geopolitical baby."

The face of the scientist.

Personnel:

Spirin AS - Scientist, biochemist, doctor of biological sciences, academician, director of the Institute for Protein Research. Filimonov VV - Candidate of Physical and Mathematical Sciences.

Calendar: 06.1984

Locations: Moscow [820] Moscow region [788]

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